http://www.blogtalkradio.com/courtney-becker-jameNext-Generation DNA Sequencing conference, January 18-20 in London, EnglandenCopyright Courtney Becker-James (C/O Blogtalkradio)Sat, 15 Jun 2019 16:30:00 GMTWed, 18 Nov 2009 15:30:00 GMTScienceBlogTalkRadio Feed v2.0http://www.blogtalkradio.com/img/btrbetalogo.gifhttp://www.blogtalkradio.com/feeds@blogtalkradio.comBlogTalkRadio.comscience,dna sequencing,medical research,abi solid,bacteria,bacteria research,conference,diagnostic,dna,gene expressionCourtney Becker-JamenoNext-Generation DNA Sequencing conference, January 18-20 in London, Englandepisodichttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/18/interview-with-dr-nicholas-h-bergman-on-dna-sequencingSciencehttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/18/interview-with-dr-nicholas-h-bergman-on-dna-sequencing/#commentshttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/18/interview-with-dr-nicholas-h-bergman-on-dna-sequencingWed, 18 Nov 2009 15:30:00 GMTinterview with Dr Nicholas H. Bergman on DNA SequencingAn interview with Dr Nicholas H. Bergman, Principal Investigator and Genomics Team Leader at the National Biodefense Analysis and Countermeasures Center, Department of Homeland Security, USA. Although more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA abundance information all provide valuable insights into gene function and regulation, but these aspects of the prokaryotic transcriptome have only been explored on a large scale in a few instances. This interview will give insight to how the National Biodefense Analysis and Countermeasures Center is using a sequencing-based approach (RNA-Seq) in assembling the first comprehensive, single-nucleotide resolution view of a bacterial transcriptome. We sampled the Bacillus anthracis transcriptome using the ABI SOLiD platform, and mapped transcript start sites and operon structure throughout the genome. Our data also provide estimates of absolute transcript abundance on a global scale, and they suggest there is significant transcriptional heterogeneity within a clonal, synchronised bacterial population. Overall, our results offer an unprecedented view of gene expression and regulation in a bacterial cell. Dr Bergman will be speaking at the IQPC Next-Generation DNA Sequencing conference in London, January 18th-20th 2010. wwww.dnaevent.com 00:45:00Courtney Becker-Jamenobacteria research,Next-Generation DNA Sequencing,genome,ABI Solid,gene expressionAn interview with Dr Nicholas H. Bergman, Principal Investigator and Genomics Team Leader at the National Biodefense Analysis and Countermeasures Center, Departhttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/13/next-generation-dna-sequencing--dr-joris-veltmanSciencehttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/13/next-generation-dna-sequencing--dr-joris-veltman/#commentshttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/13/next-generation-dna-sequencing--dr-joris-veltmanFri, 13 Nov 2009 12:00:00 GMTNext Generation DNA Sequencing - Dr Joris VeltmanAn interview with Dr Nicholas H. Bergman, Principal Investigator and Genomics Team Leader at the National Biodefense Analysis and Countermeasures Center, Department of Homeland Security, USA. Although more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA abundance information all provide valuable insights into gene function and regulation, but these aspects of the prokaryotic transcriptome have only been explored on a large scale in a few instances. This interview will give insight to how the National Biodefense Analysis and Countermeasures Center is using a sequencing-based approach (RNA-Seq) in assembling the first comprehensive, single-nucleotide resolution view of a bacterial transcriptome. We sampled the Bacillus anthracis transcriptome using the ABI SOLiD platform, and mapped transcript start sites and operon structure throughout the genome. Our data also provide estimates of absolute transcript abundance on a global scale, and they suggest there is significant transcriptional heterogeneity within a clonal, synchronised bacterial population. Overall, our results offer an unprecedented view of gene expression and regulation in a bacterial cell. Dr Bergman will be speaking at the IQPC Next-Generation DNA Sequencing conference in London, January 18th-20th 2010. wwww.dnaevent.com 00:30:00Courtney Becker-JamenoDNA,joris veltman,genomics,iqpc,sequencingAn interview with Dr Nicholas H. Bergman, Principal Investigator and Genomics Team Leader at the National Biodefense Analysis and Countermeasures Center, Departhttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/10/using-next-generation-sequencing-technology-to-study-the-complexity-of-the-bacterial-transcriptomeSciencehttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/10/using-next-generation-sequencing-technology-to-study-the-complexity-of-the-bacterial-transcriptome/#commentshttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/10/using-next-generation-sequencing-technology-to-study-the-complexity-of-the-bacterial-transcriptomeTue, 10 Nov 2009 15:00:00 GMTUsing Next Generation Sequencing Technology to Study the Complexity of the Bacterial TranscriptomeAn interview with Dr Nicholas H. Bergman, Principal Investigator and Genomics Team Leader at the National Biodefense Analysis and Countermeasures Center, Department of Homeland Security, USA. Although more than a thousand bacterial genomes have been sequenced, our understanding of bacterial transcriptomes has lagged far behind. Transcript structure, operon linkages, and absolute mRNA abundance information all provide valuable insights into gene function and regulation, but these aspects of the prokaryotic transcriptome have only been explored on a large scale in a few instances. This interview will give insight to how the National Biodefense Analysis and Countermeasures Center is using a sequencing-based approach (RNA-Seq) in assembling the first comprehensive, single-nucleotide resolution view of a bacterial transcriptome. We sampled the Bacillus anthracis transcriptome using the ABI SOLiD platform, and mapped transcript start sites and operon structure throughout the genome. Our data also provide estimates of absolute transcript abundance on a global scale, and they suggest there is significant transcriptional heterogeneity within a clonal, synchronised bacterial population. Overall, our results offer an unprecedented view of gene expression and regulation in a bacterial cell. Dr Bergman will be speaking at the IQPC Next-Generation DNA Sequencing conference in London, January 18th-20th 2010. wwww.dnaevent.com 00:45:00Courtney Becker-JamenoDNA sequencing,bacteria,virus,RNA,medical researchAn interview with Dr Nicholas H. Bergman, Principal Investigator and Genomics Team Leader at the National Biodefense Analysis and Countermeasures Center, Departhttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/06/next-generation-dna-sequencing--dr-joris-veltmanSciencehttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/06/next-generation-dna-sequencing--dr-joris-veltman/#commentshttp://www.blogtalkradio.com/courtney-becker-jame/2009/11/06/next-generation-dna-sequencing--dr-joris-veltmanFri, 06 Nov 2009 10:00:00 GMTNext Generation DNA Sequencing - Dr Joris VeltmanAn Interview with Dr Joris Veltman, Department of Human Genetics, Radboud University Nijmegen Medical Centre - Next generation sequencing has tremendous potential for clinical applications. For most diagnostic applications a targeted analysis of known disease genes is preferred at present, as this will reduce the number of sequencing errors, facilitate functional interpretation of sequence variations, and overcome limitations in computational power as well as ethical restrictions. This application is of great value for complex monogenic disorders such as blindness, deafness and mental retardation that can be caused by hundreds of genes. In this presentation I will discuss the validation of an array-based sequence capture method for medical resequencing approaches in these disorders. I will also discuss implementation of this approach in the clinical arena, which will have a profound effect on the diagnosis and genetic counselling of patients with complex monogenic disorders, relevant to all diagnostic genetics laboratories. Dr Veltman will be speaking at the forthcoming Pharma IQ conference, Next-Generation DNA Sequencing, January 17-19 in London, England.00:15:00Courtney Becker-Jamenogenetic analysis,DNA sequencing,diagnostic,conference,medical researchAn Interview with Dr Joris Veltman, Department of Human Genetics, Radboud University Nijmegen Medical Centre - Next generation sequencing has tremendous potenti