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Manohar Furtado, Ph.D. is currently consulting for Biology for Global Good LLC.
Lead R&D and Molecular Diagnostics teams at Applied Biosystems Inc., Celera Diagnostics and Life Technologies for 12 years.
Assistant Professor of Pathology and Director, Clinical Virology and Retrovirology Laboratories at Northwestern University Medical School for 7 years.
Over 15 years’ experience working in the Life Sciences domain in academic research and clinical research as a PI at the Robert H. Lurie Comprehensive Cancer Center.
Ph.D., M.Sc., B.Sc., Poona University, Biochemistry (Area: Protein structure and evolution).
70 publications in peer-reviewed journals.
Over 200 oral presentations and posters at major national and international conferences.
Two issued patents and ten pending patent applications.
2. What methods are traditionally used to detect avian influenza virus infections?
3. What is Real-time reverse transcription (RT)-PCR?
4. How do RT-PCR technologies compare with culture and traditional methods?
5. What are the limitations of liquid RT-PCR assays?
6. What is lyophilization?
7. What were the findings of the recent RT-PCR lyophilized assay trials?
Manohar R. Furtado, Ph.D.
Global Good LLC.
420 Ventura Pl, San Ramon, CA 94583
Implementation of PCR Surveillance in Food Production with Dongyu Liu, DVM, Ph.D, former Assistant Research Professor, CVM Basic Science, Mississippi State University.
Dr. Liu has authored five (5) texts on Polymearse Chain Reaction as a detection methodology for human pathogens.
He has published twenty-five (25) articles in peer-reviewed journals on topics ranging from microbiology to PCR surveillance of food for human pathogens.
In his text, "Molecular Detection of Foodborne Pathogens," he has written, "One of the most effective ways to control and prevent human foodborne infections is to implement a pathogen surveillance system. Addressing the biology, epidemiology, and pathogenesis of more than 60 microorganisms, Molecular Detection of Foodborne Pathogens demonstrates how to implement a surveillance system that uses state-of-the-art molecular techniques to quickly detect and identify bacterial, fungal, viral, and parasitic pathogens concerned."
Of his text, "Molecular Detection of Human Bacterial Pathogens," he has written, "This book represents a reliable and convenient reference on molecular detection and identification of major human bacterial pathogens; an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in bacterial characterization; and an essential textbook for undergraduate and graduate students in microbiology."
2. Traditional microbiological methods for food testing.
3. Culture methods: time/sensitivity/specificity.
4. Polymerase Chain Reaction: time/sensitivity/specificity.
5. PCR's operational functionality in a food plant.
6. Recommendations to food producers regarding PCR surviellance.
Dongyu Liu, DVM, Ph.D.
Contel Bradford is a professional of many trades--aspiring screenwriter, affiliate marketer in training and published author. He excels at writing articles about interenet technology, specializing in topics that range from email marketing and web hosting to social media and SEO. Learn more about this multi-talented man of mystery at contelbradford.com
Author Yella was born in Charlestion, SC in 1977. He grew up in Jamiaca, Queens, New York, Rochdale Village. Yella has three kids: Shahi, Radayshia and Shataysia. He's mixed of Columbian, Indian and black and is 37. Author Yella divorced and is now serving a life term for murder in which he took the rap for a charge. Now, he is fighting for his freedom of actual innocence, demanding another PCR hearing. Yella studies the teaching of the five percent Nation of Gods and Earths. Yella is striving to express his self through books so that he can give a voice to the many silenced brothers and sisters who have lost theirs in the struggle or due to it.
- "This Rahim. My truth! Get at me. The #1 spot is mine." - Author Yella
A critical look and challenge of the unproven theories, discredited research claims, fabrications, manipulations, and unfulfilled predictions surrounding the HIV = AIDS hypothesis.
After 30 years, the virus named HIV has not been proven to cause AIDS.
The evidence that a virus claimed to be HIV even exists is based on evidence that some scietists argue is insufficient.
Some scientists who acknowledge that a new retrovirus had been discovered in 1984 nevertheless are not convinced that it causes AIDS or any other adverse health effect.
There is no test for HIV that provides a reliable result for infection. In practice, diagnoses of HIV infection (HIV+) are made by assuming that two unreliable results add up to a reliable result. The truth is, that's the best they can do.
HIV infection and AIDS are treated using a mixture or cocktail of drugs of two types: nucleoside analogs and protease inhibitors. Both are toxic and eventually cause iatrogenic disease. Nucleoside analogs,such as AZT, can create AIDS symptoms. AZT has been referred to as "AIDS by prescription" by Professor Peter Duesberg, a prominent virologist from University of California-Berkeley.
Think of HIV tests and AIDS defining conditions as vehicles to entrap people into becoming lifelong pharmaceutical clients. The tab is paid with tax dollars, but people are still needed to swallow the obscenely overpriced poison pills.
Gay men, i.v. drug users, young people, pregnant women, African Americans, and the Hip Hop community are among the groups specifically targeted in campaigns urging people to "get tested."
Part 2of2 interview with David Crowe, president of Rethinking AIDS. Interview by Richard Jannaccio and Chief69.
A critical look and challenge of the unproven theories, discredited research claims, fabrications, manipulations, and unfulfilled predictions surrounding the HIV = AIDS hypothesis. After 30 years, the virus named HIV has not been proven to cause AIDS. Part 2of2 interview with David Crowe, president of Rethinking AIDS.
April 23, 2014 marks the 30th Anniversary of a day that changed the course of history. on that day, ...
Our guest is David Crowe ...
In this Special 2-part series, we will show that HIV has never been proven to cause AIDS. We will discuss what AIDS really is.You may be surprised to learn that none of the tests for AIDS--NONE of them--is approved for use in diagnosing HIV infection. etc. etc.
Every Thursday LIVE from the Bronx at 11 pm, with Hosts Chief69 and Richard Jannaccio.
in Pop Culture
This week we will be meeting with Marshall Edwards, a local Kansas City comic creator, and writer for Praire City Response, we will be talking with Marshall about the challenges, and rewards of being a comic creator in today's world. We encourage our listeners to call in live for questions for Marshall or the hosts during this show!
The Culture of Comics has longer, meta discussions about themes and art in current runs of different books. It will often contain "spoilers" in current runs and "previews" of upcoming books.
Leatherhead Opens ‘DirtyLab’ For Pathogen Challenge Testing with John Haines, Leatherhead Food Research
John has 23 years’ experience working in the clinical and food sectors, with 16 years’ service at Leatherhead working on the development, optimization and evaluation of new technologies for the rapid separation, concentration and detection of analytes of importance to food safety and quality, including foodborne bacterial pathogens, toxins, viruses, allergens and adulterated ingredients. Technologies for separation and concentration include SPE, immunoaffinity, magnetic separation, dielectrophoresis, free flow electrophoresis and molecular imprinting; and for detection include RT-PCR, immunoassay, microarray, optical biosensors and fluorescence microscopy. Current work includes the development of methods for the detection of antibiotic resistant bacteria (e.g. ESBL’s) and virus (HNov and surrogates) inactivation during food processing.
Agenda: 1. Introduction. 2. What is a human pathogen? 3. How do pathogens contaminate food products? 4. What are the methods to test for these pathogens? 5. Leatherhead’s new Pathogen Pilot Plant, also known as DirtyLab, mission. 6. Which processes deliberately contaminate food products with pathogens? 7. How will this investigate micro-organisms under selected processing conditions? 8. How does challenge testing in an environment which replicates a food production areas help? 9. How does the lab assist with industry best practices? 10. Conclusion.
Contact: John Haines Principal Scientist Food Safety Leatherhead Food Research +44 1372 822347 email@example.com http://www.leatherheadfood.com
The Use of Pathatrix Technologies in Food Testing with Melanie Patterson. Melanie is the Food Safety Consultant for Life Technologies in Europe. Her role is to understand market requirements and apply her microbiology background to identify what solutions Life Technologies can provide to better meet customer testing needs. She works closely with the commercial teams, product management and R&D. She has worked for Life Technologies for 5 years. She enjoys the frequent customer interaction and attending industry forums and events. Her background includes a BSc in microbiology and 13 years working in the food safety industry. She has worked in a contract laboratory both at the bench and helping customers with troubleshooting. She also has experience working as a support specialist across Europe. Agenda: 1. Introduction. 2. What is the goal of food testing for human pathogens? 3. Which bacterial pathogens cause the most harm? 4. What are some of the methods to detect those pathogens? 5. How does the Pathatrix technology address those challenges? 6. What is re-circulating immuno-magnetic separation (RIMS) technology? 7. How does a high volume wash enable the efficient removal of sample matrices, non-specific microorganisms and PCR inhibitors? 8. What are the advantages of Pathatrix? 9. Are there any alternatives to Pathatrix? 10. Conclusion
Melanie Patterson Senior Product Applications Specialist for Food & Safety Life Technologies http://lifetechnologies.com/foodsafety firstname.lastname@example.org +800-500-6885
The potential benefits for food producers when they engage the screening of food production for human bacterial pathogens with quantitative, real-time Polymerase Chain Reaction technologies.
AME offers the following rapid, robust and validated PCR Assays to Food Safety and production Quality Assurance/Quality Control (QA/QC) managers. The bacterial challenges AME can assist in testing for the presence/absence and quantitation are:
-E. coli species Assay
-Shigatoxin/O157 E. coli (FDA BAM method)
-Listeria species Assay
-Salmonella species Assay
The implementation of these cutting-edge PCR technologies can economically be rapidly effected in food production facitilies. Discover how AME can assist your organization in engaging these tools today.
Microbiological Systems Engineering
Test and Release Programs for Pathogen Detection in Food Production with Dr. John Murray. An academic high achiever with broad commercial experience in the rapid microbiology, diagnostics and biotechnology sector. His scientific credibility is recognised as an industry expert in sample preparation and diagnostic microbiological & pathogen detection assays for food safety, clinical and biodefense applications he honed his business and technical skills during 14 years in commercially focused R&D management and product development and validation. John established and managed the laboratory at Matrix MicroScience Ltd where a portfolio of world class antibody, enzymatic and PCR based diagnostics were developed & validated under his technical guidance.
The PATHATRIX product range became a gold standard diagnostic tool - chosen by Cadbury Schweppes Inc, Kraft Foods, the FDA, USDA clients.
2. What is a test and release program for food production?
3. Which technologies would enable food producers to screen food prior to release to the market?
4. What are the benefits of a test and release program?
5. What are the detriments of a test and release program?
6. What was your experience with the Cadbury and Kraft Salmonella screening programs?
7. What was your experience with the USDA and E. coli O157:H7 screening program?
8. What was your experience in the 2006 E. coli spinach outbreak?
9. What was your experience in the 2007 Salmonella peanut butter outbreak?
10. Which technology was employed in the 2006 and 2007 pathogen outbreak for detection?
11. What is the veracity of the statement that " you cannot test food safe?
John Murray, Ph.D.
Rapid Diagnostics for Food Safety and Clinical IVD
+44 0777 567 1735
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